Dr. Miriam Susanna Lucas-Droste

Dr.  Miriam Susanna Lucas-Droste

Dr. Miriam Susanna Lucas-Droste

Lecturer at the Department of Materials

ETH Zürich

ScopeM

HPM C 57.2

Otto-Stern-Weg 3

8093 Zürich

Switzerland

Additional information

Course Catalogue

Spring Semester 2024

Number Unit
327-2144-00L Microscopy Training Cryogenic Electron Microscopy

 

ScopeM related details

Expertise

  • Correlative light and electron microscopy (CLEM)
  • Electron microscopy for live sciences and soft materials: (cryo-)SEM, TEM, and volume EM (FIB-SEM, SBF-SEM and array tomography)
  • Establishment and adaption of respective sample preparation methods for above mentioned imaging techniques
  • 3D illustration and modelling of volume EM data

Instrument Responsibilities at ScopeM

  • Leica Freeze-Substitution (HPM A56.2)
  • PELCO BioWave and chemistry lab in general (HPM A56.1)
  • Ultramicromes
    • Leica EM FC6 Cryo (HPM C58)
    • Leica EM UC7 (HPM C56)
    • Ultracut E (HPM C56)
  • TFS Quanta 250 3VIEW (HPT C10)
  • TFS Morgani 268 (HPM A 51.1)
  • Staining lab (HPM A58.2)

Biography

Publications

Heiligenstein, X. & Lucas, M. S. (2022): One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM. Front. Cell Dev. Biol (10); http://dx.doi.org/10.3389/fcell.2022.866472

Reyes, C., Sajó, Z., Lucas, M. S., et al. (2022): Cocultivation of White-Rot Fungi and Microalgae in the Presence of Nanocellulose. Microbiol. Spectr. (10); http://dx.doi.org/doi:10.1128/spectrum.03041-22

Tran, H. T., Lucas, M. S., Ishikawa, T., et al. (2021): A Compartmentalized Neuronal Cell-Culture Platform Compatible With Cryo-Fixation by High-Pressure Freezing for Ultrastructural Imaging. Front. Neurosci. (15); http://dx.doi.org/10.3389/fnins.2021.726763

Lucas, M. S., Gunthert, M., Bittermann, et al. (2017): Correlation of live-cell imaging with volume scanning electron microscopy. Methods Cell Biol. (140); http://dx.doi.org/10.1016/bs.mcb.2017.03.001

Burkhardt, M. A., et al. (2017): Clot-entrapped blood cells in synergy with human mesenchymal stem cells create a pro-angiogenic healing response, Biomater. Sci.(5); http://dx.doi.org/10.1039/C7BM00276A

Spyrogianni, A., Herrmann, I. K., Lucas, M. S., et al. (2016): Quantitative analysis of the deposited nanoparticle dose on cell cultures by optical absorption spectroscopy. Future Med. (11); http://dx.doi.org/10.2217/nnm-2016-0243

Walter, K. M., et al. (2014): Hif-2alpha promotes degradation of mammalian peroxisomes by selective autophagy. Cell Metab. (20); http://dx.doi.org/10.1016/j.cmet.2014.09.017

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