Zeiss LSM 780
Brief description:
With the Zeiss 780 laser scanning confocal microscope you can perform automated longterm multi-position life cell experiments. The system is equipped with a motorized X-Y stage (Märzhäuser 130 x 85 mot P), a piezo stage for fast focussing and with an incubator box (temperature, CO2).
The system is further equipped with:
- 2 conventional PMTs and a high sensitive multiarray 32PMT GaAsP detector for fast spectral scanning
- a high NA long working distance 40x water immersion objective (LD C-Apochromat 40x/1,1 W Korr M27) NA: 1,1 WD: 620 µm @ coverslip thickness 0.17 mm) which allows confocal imaging of several cell layers
- the Zeiss hardware autofocus system (definite focus)
- AxioCam MRm Rev.3 FireWire as well as GFP, CFP, YFP, DAPI, RFP and Cy3.5 filter sets for widefield microscopy
Microscope:
- Zeiss AxioObserver.Z1
Ilumination:
- HXP 120 C (visual observation+widefield)
- laser lines:
- Diode 405 nm
- Argon: 458, 477, 488, 514 nm
- Solid state: 561 nm
- HeNe: 594 nm
- HeNe: 633 nm
Objectives:
- 10x 0.3NA EC Plan-Neofluar Ph1 M27
- 20x 0.5NA EC Plan-Neofluar Ph2 M27
- 40x 1.1NA Water LD C-Apochromat Korr M27 (DIC)
- 63x 1.4NA Oil Plan-Apochromat DIC M27
Camera / detectors:
- 2 PMTs, 1 32PMT GaAsP detector, 1 transmission PMT
- AxioCam MRm Rev.3 FireWire (1388x1040 pixel; pixel size 6.45 µm x 6.45 µm)
Software:
- ZEN Black 2012
- You can download the an offline ZEN 2012 light version via www.zeiss.com/zen-lite onto your PC (no Mac version available).
Incubator:
- The system is equipped with an full box type incubation including stage incubation and CO2 gassing (Pecon).
Fluorescence settings:
- for visual observation GFP, CFP, YFP, DAPI, RFP and Cy3.5 filter sets are available