Zeiss LSM 780 upright

Brief description:

The Zeiss 780 upright laser scanning confocal microscope as an upright setup can be used for multicolour fluoerescence imaging as well as for surface analyzis/topography application. The system is equipped with a motorized X-Y stage (135x80) and a stage top incubator (Pecon; temperature, CO₂). The system is further equipped with a tunable chameleon multi photon laser, 2 conventional PMTs and a high sensitive multi array GaAsP detector which can be used for fast lambda scans. For surface data analyzis the microscope computer is equipped with the Confomap® software.

Microscope:

• Zeiss AxioImager.Z2
  

Ilumination:

• HXP 120 V (visual observation)
• laser lines:
  • Diode 405 nm
  • Argon: 458, 488, 514 nm
  • Solid state: 561 nm
  • HeNe: 594 nm
    
  • HeNe: 633 nm
  • Chameleon MP laser: 705–980 nm

Objectives:

• 10x 0.3NA EC Plan-Neofluar Ph1 M27
• 20x 0.8NA Plan-Apochromat M27
• 40x 0.75NA EC Plan-Neofluar Ph2 M27
• 40x/0.75NA W N-Achroplan M27 (water dipping lense)
  
• 40x 1.3NA Oil Plan-Neofluar DIC M27
• 63x 1.4NA Oil Plan-Apochromat DIC M27

Camera / detectors:

• 2 Quasar PMTs (fluorescence), 1 32PMT GaAsP detector, 1 transmission PMT

Software:

• ZEN Black 2012
• Confomap

Incubator:

• The system is equipped with a stage top incubator including temperature control and CO₂ (Pecon).

Fluorescence settings:

• for visual observation DAPI, GFP, mCherry and Cy5 filter sets are available