Zeiss Lattice Lightsheet

Brief description:

The Zeiss lattice lightsheet is an inverted microscope platform to image live (and fixed) samples in standard sample carriers (with coverslip bottom #1.5), f.i. 35 mm glass bottom dishes, Labtek chambers, IBIDI slides.

The system is equipped with 3 laser lines (488 nm, 561 nm and 640 nm), two Hamamatsu Fusion sCMOS camera and a 48x/1.0NA detection objective. The lattice lightsheet microscope is fully incubated for live cell experiments. Acquisition and data processing is integrated in ZEN Blue.

Major advantages of Zeiss lattice lightsheet microscopy are:

• low photobleaching
• low phototoxicity
• fast volume acquisition speed
• easy lightsheet adjustment and calibration

Experiments aiming for the lattice lightsheet microscope are:

• Long time recording (several hours up to days) of cellular and subcellular dynamics
• Shorter recordings at highest volume speeds
• Single plane acquisition at high frame rates
  

Ilumination:

• laser lines: 488 nm, 561 nm, 640 nm
  

Objectives:

• 48x 1.0NA
  

Camera / detectors:

• sCMOS Hamamatsu Orca Fusion C14440-20UP

Software:

• ZEN blue