Zeiss CD7-LSM

Manufacturer:
Zeiss

Model name:
Celldiscoverer 7 LSM 900 AiryScan 2

Location:
HPM C46

Phone:
37681

external pageDirect booking link to PPMS

Instrument contact:

Michael Stebler
  • HPM C 44
  • +41 44 633 76 89
  • vCard Download

ScopeM
Otto-Stern-Weg 3
8093 Zürich
Switzerland

Enlarged view: CD7-LSM

Brief description:

The Zeiss Celldiscoverer 7 is an automated fluorescent wide-field and confocal microscope capable of imaging microplates and microscopy slides. It is equiped with an automated water-immersion system for the 50x objective. It is integrated in a robotic system with hotels at room-temperature and an automated incubator. That allows to automatically image several plates in sequence or make long-term live-cell experiments.

Microscope:

  • Zeiss Celldiscoverer 7

Contrast Methods:

  • Adaptive Phase Gradient Contrast (PGC), Widefield Fluorescence

Ilumination (visual):

  • Calibri.2 LED Illumination: 385/420/470/520/567/590/625 nm
  • LSM 900 Lasermodul LM URGB (D)
    • Diodelaser 405 nm, 5 mW,
    • Diodelaser 488 nm, 10 mW,
    • Diodelaser (SHG) 561 nm, 10 mW,
    • Diodelaser 640 nm, 5 mW,

Beam Splitter:

  • RQFT 405+493+575+653
  • RTFT 450+538+610
  • RTFT 405+493+610

Emission Filters:

  • QBP 425/30+514/30+592/25+709/100
  • TBP 467/24+555/25+687
  • TBP 425/30+524/50+688
  • 431/28
  • 515/30
  • 598/25

Objectives:

  • PlanApochromat 5x/0.35/WD: 5.1 mm
  • PlanApochromat 20x/0,7/WD: 2.2 mm
  • PlanApochromat 20x/0,95/WD: 0.76 mm
  • PlanApochromat 50x/1,2 Water | Autoimmersion/WD: 0.84 mm

These objectives are used in combination with a 0.5X, 1x and 2x optovar lens. Possible magnifications: 2.5x, 5x, 10x, 20x, 25x, 40x, 50x, 100x.

Camera / detectors:

  • Axiocam 712 mono
  • LSM 900 Scanmodul: 2 Gallium Arsenide Phosphid-PMT (GaAsP-PMT)
  • LSM 900 Airyscan 2 Detector (32 GaAsP-PMT array for super-resolution)

Incubation:

  • The microsoce is equipped with a temperature control and CO2 gassing system.

Software:

  • ZEN Blue

Key features:

  • Integrated into a robotic system with an incubator, allows to re-image plates over a extendet time period
  • Hardware based autofocus
  • Automated water immersion (50x)
  • Integrated image analysis allowing for 'guided acquisition'
  • Can be controlled by Python macros
  • Superresolution (Airyscan 2)
  • Barcode recognition
  • Automatic calibration routines  
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