Tomocube HT-2H QPI

Brief description:

Holotomographic Microscopy utilize optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate biological cells and tissues. ODT reconstructs the 3D refractive index (RI) distributions of live cells using a dynamic micromirror device (DMD) and by doing so, provides structural and biochemical information about the cell including dry mass, morphology, and dynamics of the cellular membrane. In addtion, the HT-2H is able to perform fluorescence microscopy.

Microscope:

• Tomocube HT-2H

Ilumination:

• Phase imaging: 532 nm diode laser
• Fluorescence : 3 LEDs, λ_center = 385 nm, 470 nm, 570 nm 

Objectives:

• 60X NA=1.2, water immersion 

Key features:

• Label-free imaging
• Refractive index measurement, Quantiative phase imaging
• Full motorized stage (X, Y, Z axis)
• Live Cell Imaging : Temperature, CO₂ controller

Technical Specification:

• Lateral resolution: 110 nm
• Axial resolution 345 nm 
• FOV: 80 μm DOF: 40 μm
• Time resolution: 150 fps (2D), 2.5 fps (3D)
• Max exposure time: 1sec

Software:

• Tomostudio 3.3.3 (Tomocube)
• Tomosticher

Link:

• external pageTechnology and product detailcall_made

Fluorescence settings: