Visitron Spinning Disk

Brief description:

This new Nipkow spinning disk setup is equipped with the latest Yokogawa Confocal Scanner Unit CSU-W1-T2. It contains two interchangeable spinning disks (pinhole diameter 25 μm for 20x/40x objectives and 50 μm for 60x/100x objectives) and can also be used for simple widefield imaging.  Due to the new disk design it almost reachs the z-resolution of a point scanning confocal. It is equipped with 2x EMCCD Andor iXon Ultra cameras (1024x1024 pixel, 13x13 μm pixel size) that allows big field-of-views and fast and sensitive live cell imaging even in a dual camera mode.

In addition, it is also equipped with a point scanner for FRAP and a LASER ablation module (iLas Pulse, Roper Scientific) for doing precise FRAP and ablation experiments. It has an full box incubator for temperature and CO₂,  a LUDL BioPrecision2 stage with Piezo Focus and XY linear encoder and filter combinations for fast multicolor acquisition. It has also a hardware autofocus system (Nikon Perfect Focus System). The two EMCCD cameras allows for simultaneous acquisition of combinations of blue and yellow, green and red, and blue and far red fluorophores.

Microscope:

• Nikon Eclipse T1 (inverse)

Contrast Methods:

• Brightfield, Widefield Fluorescence, Spinning Disk Confocal Fluorescence (2 disks, 25um and 50um pinhole size)

Ilumination (visual):

Lumencor SpextraX Light Engine

• 390/18
• 438/24
• 475/28
• 513/17
• 549/15
• 575/25
• 632/22

Ilumination/FRAP (LASER):

• DPSS 405 nm 120 mW
• DPSS 445 nm 100 mW
• DPSS 488 nm 200 mW
• DPSS 515 nm 100 mW
• Diode 561 nm, 200 mW
• 640 nm, 150 mW

Ablation LASER:

• 355 nm, 400 ps pulse duration, 20 000 Hz repetition rate, peak power 2kW, pulse energy 1μJ

Objectives:

• 10x 0.45 CFI Plan Apo λ
• 20x 0.75 CFI Plan Apo VC
  
• 60x 1.2 CFI Plan Apo VC Water
  
• 60x 1.4 CFI Plan Apo λ Oil
  
• 100x 1.4 CFI Plan Apo Oil
  
• 100x 0.5 - 1.3 Iris CFI S Fluor (Ablation)

Camera / detectors:

• 2x EMCCD Andor iXon Ultra, 1024x1024 pixel, 13x13 μm pixel size

Incubation:

• The system is equipped with an full box type incubation and CO₂ gassing (Okolab). So far it can handle 35 mm dishes, LabTek chambered coverglasses, Ibidi slides, 96 well plates
  

Software:

• VisiVIEW ("Metamorph") including Scan Slide, Multiwell Scanning, FRAP and Laser ablation module

Fluorescence settings reflector turret:
(only for visual inspection)