Olympus MM
Brief description:
This wide field fluorescence microscope can be used for automated long-term, multi-position live cell imaging. The setup includes a motorized X-Y stage, and a box-type incubator (temperature, CO2). LED (Lumencore SpectraX) illumination in combination with multiband chromatic mirrors and a fast emission filterwheel allow for fast multichannel acquisition. It is equipped with the ZDC2 (Z-drift compensator) - a "hardware auto-focus" solution from Olympus. There are also the options for phase contrast imaging, DIC imaging and dark-field imaging.
A full offline version of Metamorph is installed on Image Processing Computer 2 Windows 7 in HPI F17 for further analysis of images.
Microscope:
- Olympus IX 83 (inverted)
Ilumination:
- LED (Lumencor SpectraX)
Objectives:
- 10x NA=0.3: Air, UPLANFLN, Ph1
- 40x NA=0.6: Air, LUCPlanFLN, Ph2
- 40x NA=1.3: Oil, UPLFLN40XO, DIC (BFP1)
- 60x NA=1.42: Oil, PLAPON60XO, DIC (BFP1)
- 100x NA=1.40: Oil, UPLSAPO100XO, DIC
- 100x NA=1.40: Oil, UPLSAPO100XOPH, Ph3
Optional
- 4x NA=0.16: Air, UPLSAPO
- 60x NA =1.25 (Iris 0.65–1.25): Oil, UPlanFLN
- 100x NA=1.3 (Iris 0.6–1.3): Oil, UPlanFLN
Contrast methods:
- Phase contrast imaging (10X, 40X, 100X)
- DIC imaging
- Dark-field imaging (transmission mode)
Camera / detectors:
- Hamamatsu ORCA-Flash 4.0 V2 (sCMOS, 2048 x 2048 pixels, pixel size 6.5 μm x 6.5 μm)
Software:
- Metamorph 7.10.1 (Molecular Devices)
Key features:
- Live Cell Imaging : Temperature, CO2 controller, Hardware autofocusing for long-term stable focusing
- Phase contast imaging, DIC imaging, Transmitted darkfield imaging