Nikon MMI Microdissection
Brief description:
This microscope enables you to perform precise, non-contact laser based micromanipulation experiments The MMI (Molecular Machines & Industries ) CellCut facilitates precise and contamination-free dissection of cell clusters, single cells or subcellular compartments from various types of tissues including fresh frozen, paraffin embedded and archived slides, cytospins, smears and even living cells. The system is operated by the MMI software.
Additionally, you are also able to do high resolution imaging of fixed stained and unstained samples and it is also possible to perform complex live cell imaging experiments. The "imaging functionality" is controlled by the Software NIS-Elements (Nikon) which allows multichannel, multiposition, time lapse imaging and complex experiments.
Microscope:
- Nikon Ti2-E (inverse) with motorized emission filter wheels, a quadband dichroic mirror (DAPI/FITC/TRITC/CY5) and a tripleband dichroic mirror (CFP/YFPmCherry)
Ilumination:
- Spectra III light engine (8 wavelenghts): 390/22, 440/20, 475/28, 510/25, 555/28, 575/25, 637/12, 748/12
- laser for cutting: 355 nm pulsed UV laser (1 μJ, 2 kHz, 500 ps)
Objectives:
- CFI Plan Apochromat 4x NA 0.2 WD 20 mm (imaging)
- CFI SPlan Fluor ELWD 20x C NA 0.45 Ph1 WD 8.2–6.9 mm (Microdissection and imaging)
- CFI SPlan Fluor ELWD ADM 40x C NA 0.60 Ph2 WD 3.6–2.8 mm (Microdissection and imaging)
- CFI Plan Apochromat Lambda 60x Oil NA 1.4 WD 0.13 mm (Imaging, DIC)
- CFI Plan Apochromat IR 60x C Water NA 1.27 WD 0.17 mm (Imaging)
- CFI SR HR Plan Apochromat Lambda S 100x C Sil NA 1.35 WD 0.3 mm (Imaging, DIC)
Camera:
- Orca Fusion BT (2304 x 2304 pixels, 6.5 μm x 6.5 μm), Fluorescence (Microfissection and imaging)
- MMI VCXU23c colour camera (1920 x 1200 pixels, 5.86 μm x 5.86 μm), Colour BF Camera, (Microdissection and imaging)
Software:
- For Laser Microdissection: MMI CellCut
- For imaging: NIS-Elements
Contrast methods:
- Brightfield, Phase Contrast, DIC, Widefield Fluorescence
Incubation:
- The system is equipped with an OkoLab box type incubation system including CO2 and temperature control.
Manuals:
- DownloadMMI CellCut Manual (PDF, 25 MB)vertical_align_bottom
- DownloadEmbedding an freezing (PDF, 862 KB)vertical_align_bottom
- DownloadCryosectioning (PDF, 644 KB)vertical_align_bottom
- DownloadParaffine Embedding (PDF, 630 KB)vertical_align_bottom
- DownloadParaffine Sectioning (PDF, 660 KB)vertical_align_bottom
- DownloadGeneral Staining (PDF, 558 KB)vertical_align_bottom
- DownloadHE-Staining (PDF, 721 KB)vertical_align_bottom
- DownloadWorking with RNA (PDF, 524 KB)vertical_align_bottom
- DownloadZeiss_LabProtocol_RNA (PDF, 564 KB)vertical_align_bottom
- DownloadZeiss RNA Paraffine (PDF, 482 KB)vertical_align_bottom
- DownloadZeiss RNA Frozen (PDF, 161 KB)vertical_align_bottom
- DownloadZeiss LabProtocol DNA (PDF, 7.3 MB)vertical_align_bottom
- DownloadZeiss LabProtocol Proteins (PDF, 7.2 MB)vertical_align_bottom
- DownloadZeiss LabProtocol FISH (PDF, 395 KB)vertical_align_bottom
- DownloadZeiss LabProtocol Chromosomes (PDF, 728 KB)vertical_align_bottom
- DownloadMMI Chromosomes (PDF, 546 KB)vertical_align_bottom
- DownloadMMI Smears and Cytospins (PDF, 579 KB)vertical_align_bottom
- DownloadZeiss LabProtocol Fluorescence (PDF, 407 KB)vertical_align_bottom
- external pageNikon JOBScall_made
Fluorescence settings:
For visual observation DAPI-like, Alexa488-like and Aexa568-like filter sets are available.