Leica stereo

Brief description:

This stereomicroscope/zoom microscope combination is equipped both for fluorescence imaging and for transmission microscopy (brightfield, darkfield, Rottermann contrast). Moreover the DFC550 colour camera can be used both for real colour imaging and for fluorescence microscopy.

The system is fully motorized (focus, zoom, stage) allowing easy multipositioning and automated tile scan experiments.

Microscope:

• Leica M205 FA

Ilumination:

• fluorescence: X-Cite 200DC lamp with 3 mm light guide
• transmission:    
  
  • epi: ring LED illumination
  • dia: LED capable of brightfield, Rottermann contrast and darkfield illumination              

Objectives:

• stereo (stereoview): Planapo 5x/0.5 LWD, magnification range: 32x–642x
• zoom (acquisition): Planapo 1x, magnification range: 8x–160x
• optional: Planapo 2x with correction collar for aqueous samples

Camera

• Leica DFC550 CCD colour camera, operating both in colour and b&w mode

Software:

Leica LAS X software, available modules:

• multi-channel acquisition
• time-lapse
• z-control and SW autofocus (z-stack + software autofocus)
• mark and find (multiposition)
• stitching (tile scan)
  

Fluorescence settings:

Widefield epifluorescence filtersets:

• equipped: DAPI , Alexa488, Texas Red
• optional: Cy5