TFS CorrSight
Brief description:
The FEI CorrSight is a purpose-built to support correlative experiments. It provides automation on the first steps in a correlative experiment. Considering how different the sample preparation protocols are, FEI CorrSight offers a variety of imaging modes and ensures compatibility with a variety of samples
Microscope:
- FEI CorrSight
Ilumination:
- Xe-lamp light source for Widefield and Structured Illumination
- SpinningDisk confocal: 4 laser line combiner (405/488/561/640 nm)
Objectives:
- 5x 0.16NA Plan-Neofluar
- 20x 0.8NA Plan-Apochromat
- 40x 0.9NA Plan-Neofluar
- 63x 1.46NA alpha Plan-Appochromat Korr M27, TIRF (optional)
- 40x 1.1NA W C-Apochromat
- 100x 1.45NA alpha Plan-fluar
Camera / detectors:
- Hamamatsu ORCA-Flash 4.0 v2 (widefield)
- Hamamatsu Orca R2 (spinning disk)
Software:
- FEI LiveAcquisition
- FEI MAPS 2.1
Key features:
- One of the specific design features of FEI CorrSight is a truly static sample stage. This allows uncompromised compatibility with different sample environments and peripherals in correlative experiments as well as optimal support for a multitude of applications. A high-end fluorescence microscope is moved precisely to scan the complete sample, allowing for high-speed image acquisition.
- CorrSight Cryo-Module - Sample holder system for correlative light and electron microscopy of vitrified samples:
- The cryo chamber has two positions for FEI AutoGrids (robust sample carrier which protects a TEM grid that contains the sample against deformation and/or damage by providing a strengthening outer rim). The AutoGrids are pre-mounted on a shuttle for quick and safe exchange.
- Transmission, widefield fluorescence and spinning disk confocal imaging modes can be used for samples in cryo conditions in combination with any of the available air objectives (5x 0.16 NA, 20x 0.8 NA, 40x 0.9 NA)