Olympus FluoView3000

Brief description:

This confocal Olympus FluoView 3000 microscope consists of  an inverted stand equipped with a live cell incubation chamber (Tokai Hit) as well as a resonant scanner for fast confocal live cell applications.

Microscope:

Olympus IX83 (inverse) fully motorized stage with fluorescence filter sets for DAPI, AF488 and AF568 (for conventional epi-illumination).

Ilumination:

• solid state laser lines: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• LED based (CoolLED pE300white) unit for conventional epi-illumination for visual observation

Objectives:

• 10x 0.3NA, UPlanFLN PH1
  
• 20x 0.75NA, UPlanSApo
  
• 30x 1.05NA, Silicon UPlanSApo
  
• 60x 1.3NA, Silicon UPlanSApo
  
• 60x 1.35NA, Oil UPlanSApo
  

Detectors:

• 4 GaAsP PMTs (spectral detection option) and 1 transmission PMT

Stage:

• Olympus IX3-SSU motorized XY stage, travel range 114 x 75 mm

Hardware outofocus:

• Z-drift compensator ZDC-2

Incubator:

• Tokai Hit STXG-IX3WX-SET, Thermobox + stage top incubator, temperature controller, gas mixer for CO₂
  
• sample adaptors for galss slides, chambered coverglass, chamberslide 35 mm dishes, 50/60 mm dishes, well plate
  

Software:

• FV3000 system software (FV31S-SW), CellSens software
  

Contrat methods:

• Brightfield, DIC, Confocal Fluorescence