Nikon Spinning Disk SoRa

Brief description:

This new Nipkow spinning disk setup is equipped with the latest Yokogawa Confocal Scanner Unit CSU-W1-T2 SoRa. It contains two interchangeable spinning disks

1. Disk 1 format: 50 µm pinholes @ 500 µm spacing, dual disk technology for normal spinning disk imaging
2. Disk 2 format: 50 µm pinholes (SoRa) @ 500 µm spacing, dual disk technolog for "super resolution imaging" XY resolution ~120–150 nm)

(see external pageherecall_made for more details)

Due to the new disk design it almost reachs the z-resolution of a point scanning confocal. It is equipped with 2x back illuminated sCMOS Hamamtasu Orca Fusion BT cameras (2304 x 2304 pixel, 6.5 x 6.5 μm pixel size) that allows big field-of-views and fast and sensitive live cell imaging even in a dual camera mode.

In addition, it is also equipped with a point scanner for FRAP for doing precise FRAP and ablation experiments. It has an full box incubator for temperature and CO₂, a Piezo z-​stage (Mad City Labs Nano-​Drive, 200 µm, with triggering) and filter combinations for fast multicolor acquisition. It has also a hardware autofocus system (Nikon Perfect Focus System). The two sCMOS cameras allows for simultaneous acquisition of combinations of blue and yellow, green and red, and blue and far red fluorophores.

The system can also be used for simple widefield imaging.

Furthermore, the system is equipped with a point scanning  "confocal arm", using the new technology of external pageRe-​scancall_made with the external pageRCM systemcall_made from confocal.nl.

Microscope:

• Nikon Eclipse Ti2 (inverse)

Contrast Methods:

• Brightfield, Phase Contrast (60x/100x), Widefield Fluorescence, "normal" Spinning Disk Confocal Fluorescence, "super resolution" Spinning Disk Confocal Fluorescence

Ilumination (visual):

• Lumencor SpextraX Chroma: 395, 440, 470, 508, 555, 640 nm
  
• Ilumination/FRAP (LASER):
  • 405 nm, 180 mW
  • 445 nm, 100 mW
  • 488 nm, 200 mW
  • 515 nm, 150 mW
  • 561 nm, 200 mW
  • 647 nm, 140 mW

Objectives:

• 10x 0.45 CFI Plan Apo λ
• 20x 0.75 CFI Plan Apo λ
  
• 40x 1.25 CFI Plan Apo λ SC Silicone
  
• 60x 1.2 CFI Plan Apo VC Water
  
• 100x 1.45 CFI Plan Apo Oil
  

Camera / detectors:

• 2x Hamamtasu Orca Fusion BT cameras (2304 x 2304 pixel, 6.5 x 6.5 μm pixel size)

Incubation:

• The system is equipped with an full box type incubation and CO₂ gassing (Okolab). So far it can handle 35 mm dishes, LabTek chambered coverglasses, Ibidi slides, 96 well plates
  

Software:

• Nikon NIS Elements: The microscope can run all common types of live-​cell experiment, combining time lapse, multi-​position (XYZ), multi-​channel and stitching (large image) functionalities. In addition, it can be custom-​scripted to perform more complex and/or ‘smart’ imaging routines (JOBS). Combined with the larger field-​of-view (FOV) spinning disk unit and triggered piezo Z-​stage, the system is particularly suitable for faster and/or higher throughput confocal imaging.

 
Fluorescence settings reflector turret (only for visual inspection):