Horiba Raman

Brief description:

This Raman instrument is ideally suited for both micro and macro measurements, and offer advanced confocal imaging capabilities in 2D and 3D. The instrument is connected with upright and inverted microscope so that variety of sample could be measured. A variety of lasers for Raman, LED illumunation for fluorescent imaging and optical tweezer are available as well.     

Raman system & microscope:

• Raman: LabRAM HR Evolution UV-VIS-NIR 
• Nikon Ti-E (inverted)
• Olympus BX (upright) 

Excitation source:

• Available Lasers (Raman):
  • 325 nm (25 mW):  HeCd laser, KIMMON 
    
  • 532 nm (1500 mW): Nd:Yag laser, Cobolt Samba^TM cw single frequency laser
  • 660 nm (100 mW): Laser Quantum Ventus 660
  • 785 nm (300 mW): High power signel frequency diode laser, Toptica XTRA II
  • Optical trapping laser 1064 nm (4000 mW): Nd:Yag laser, LaserQuatum opus 1064
• Fluorescent lightsource (CoolLED pE4000)
  

Objectives:

• inverted microscope:
  • 10x, Nikon PlanFluor, NA=0.3     
  • 20x, Nikon PlanApo, NA=0.75     
  • 40x, Nikon PlanApo, NA=0.95  
  • 60x, Nikon
    
  • 100x, Nikon   
    

• upright microscope
  •  10x, Olympus MPlanN, NA=0.25, WD=10.6 mm
  •  40x, Thorlabs LMU-40X UVB,  NA=0.49, WD=0.8 mm (for UV)
  •  50x, Olympus LMPlanFLN, NA=0.5, WD=10.6 mm
  • 100x, Olympus MPlanN, NA=0.9, WD=0.21 mm

Contrast methods:

• SWIFT^TM: It offers confocal Raman mapping with CCD integration times down to 1ms mper point and below
• DuoScan^TM: a confocal imaging mode, with high precision, ultra-fast rastering mirrors creating variable size laser macro-spot, and also alllowing nano-step mapping from deep UV to NIR (inverted microscope only)

Camera / detectors:

• Horiba Synapse EM-CCD (Front illuminated CCD, 1600 x 200 pixels, pixel size: 16 μm x 16 μm)  
• Horiba Synapse Back illuminated deep depleted CCD: (1024 x 256 pixels, pixel size: 26 μm x 26 μm)
• Fluorescent microscopy: Hamamatsu ORCA-Flash 4.0 V2 (sCMOS, 2048 x 2048 pixels, pixel size 6.5 μm x 6.5 μm)
• Visualization: IDS uEYE color camera

Grating for Raman spectroscopy:

Default  

• 300 gr/mm (Blaze 600 nm) ruled grating blased at 600 nm  
• 1800 gr/nm (Blaze 500 nm)
  

Optional  

• 300 gr/mm (Blaze 1000 nm) ruled grating blased at 1000nm (spectral range 600–4000 nm)
  
• 600 gr/nm (Blaze 500 nm)
• 1800 gr/nm (Blaze 500 nm) 
• 2400 gr/mm (blaze 330 nm) holographic grating blased at 330nm (specral range 190–650 nm) 

Software:

• Raman : Labspec 6.5, Multiwell, EasyNav
• Postprocessing: Solo + MIA academic, Multivariate Analysis
• Database: KnowItAll
  
• Fluorescent microscopy : Micromanger 

Key feature:

• Spectral range: UV-VIS-NIR: 200–2200 nm
• Raman microscopy for both upright and inverted microscope
• Fluorescent microscopy for both upright and inverted microscope
• Optical tweezer for inverted microscope

Fluorescence settings: